| Tetreuaresta latipennis (Townsend, 1893) |
| Tetreuaresta rufula (Wulp, 1900) |
| Tetreuaresta timida (Loew, 1862) |
| Tomoplagia Coquillett, 1910 |
| Tomoplagia cressoni Aczél, 1955 |
| Tomoplagia obliqua (Say, 1830) |
| Toxotrypana Gerstaecker, 1860 |
| Toxotrypana curvicauda Gerstaecker, 1860 |
| Toxotrypana furcifera (Bigot, 1884) |
| Toxotrypanini Hancock, 1986 |
| Trupanea Schrank, 1795 |
| Trupanea actinobola (Loew, 1873) |
| Trupanea arizonensis (Malloch, 1942) |
| Trupanea bisetosa (Coquillett, 1899) |
| Trupanea conjuncta (Adams, 1904) |
| Trupanea digrammata (Hering, 1947) |
| Trupanea eclipta Benjamin, 1934 |
| Trupanea femoralis (Thomson, 1869) |
| Trupanea imperfecta (Coquillett, 1902) |
| Trupanea mallochi (Hering, 1940) |