| Megachile (Sayapis) slevini Cockerell, 1924 |
| Megachile (Sayapis) zapatlana Cresson, 1878 |
| Megachile (Tylomegachile) Moure, 1953 |
| Megachile (Tylomegachile) simplicipes Friese, 1921 |
| Megachile (Tylomegachile) tiburonensis Cockerell, 1924 |
| Megachile (Tylomegachile) toluca Cresson, 1878 |
| Megachile (Xanthosarus) Robertson, 1903 |
| Megachile (Xanthosarus) agustini Cockerell, 1905 |
| Megachile (Xanthosarus) cochisiana Mitchell, 1934 |
| Megachile (Xanthosarus) comata Cresson, 1872 |
| Megachile (Xanthosarus) gemula Cresson, 1878 |
| Megachile (Xanthosarus) perihirta Cockerell, 1898 |
| Megachile (Xanthosarus) subfortis Mitchell, 1936 |
| Megachile (Xanthosarus) valida Smith, 1879 |
| Megachile aurorea Friese, 1916 |
| Megachile brachysoma Cockerell, 1924 |
| Megachile estebana Cockerell, 1924 |
| Megachile knabi Cockerell, 1919 |
| Megachile peruviana Smith, 1879 |
| Megachile rhodopus Cockerell, 1896 |