| Acanthotrema Travassos, 1928 |
| Acanthotrema hancocki (Martin, 1950) Lafuente, Roca & Carbonell, 2000 |
| Ascocotyle Looss, 1899 |
| Ascocotyle ampullacea Miller & Harkema, 1962 |
| Ascocotyle angrensis Sogandares-Bernal & Lumsden, 1963 nec Travassos, 1916 |
| Ascocotyle chandleri Lumsden, 1963 |
| Ascocotyle diminuta Stunkard & Haviland, 1924 |
| Ascocotyle felippei Travassos, 1928 |
| Ascocotyle gemina Font, Heard & Overstreet, 1984 |
| Ascocotyle leighi Burton, 1956 |
| Ascocotyle leighi Ramos-Ramos, 1994 nec Burton, 1956 |
| Ascocotyle longa Ransom, 1920 |
| Ascocotyle macrostomus (Robinson, 1956) Sogandares-Bernal & Lumsden, 1963 |
| Ascocotyle mcintoshi Price, 1936 |
| Ascocotyle megalocephala Price, 1932 |
| Ascocotyle mollinesicola (Sogandares-Bernal & Bridgman, 1960) Sogandares-Bernal & Lumsden, 1963 |
| Ascocotyle nana Ransom, 1920 |
| Ascocotyle nunezae Scholz, Vargas-Vázquez, Vidal-Martínez & Aguirre-Macedo, 1997 |
| Ascocotyle pindoramensis (Travassos, 1929) Simoes, Scholz, Santos-Barbosa & Portes-Santos, 2006 |
| Ascocotyle sexidigita Martin & Steele, 1970 |